Gepard (German: "cheetah", Backronym for "GEnome PAir - Rapid Dotter") allows the calculation of dotplots even for large sequences like chromosomes or bacterial genomes. Reference: Krumsiek J, Arnold R, Rattei T. Gepard: A rapid and sensitive tool for creating dotplots on genome scale. Bioinformatics 2007; 23(8): 1026-8. PMID: 17309896
Local comparison two of nucleotide or amino acid sequences from user-specified files. Batch dotplot functionality provided by command line access to Gepard.
Gepard requires the Java Runtime Environment Version 5.0 or later (http://www.java.com/download/). It has been tested on the following operating systems:
Please download the jar file if you want to run gepard on your computer (Java needs to be installed). On MacOS, nagivate to your Downloads folder and open the jar file via right-click. Confirm the execution of this program.
The source code is available in our GitHub repository gepard.
An offline version of the tutorial is included in the download package and in the source code.
Gepard utilizes suffix arrays for rapid heuristic dotplot calculation. For large dotplots it searches exact word matches of a certain length (10 by default) from one sequence in the suffix array of the other sequence. As an arbitary word is found in log(n) time within a suffix array this method reduces complexity of the dotplot calculation from O(m*n) to O(m * log n) (where n is the length of the longer, m the length of the shorter sequence). For small dotplots the classical window-based dotplot calculation is utilized.
The program uses the "Skew" algorithm to calculate the suffix arrays. This algorithm is very memory-intense so Gepard might require a large amount of available memory. Unfortunately, the Java VMs for all operating systems have to be given the maximum amount of available memory at startup. This is why there are different startup scripts for different machines. The following table shows the approximate maximum sequence size (assuming a self-plot) for each memory setting. This includes both suffix array and dot matrix calculation.
|256MB||~10 million base pairs|
|512MB||~20 million base pairs|
|1024MB||~40 million base pairs|
Gepard supports the program "mkvtree" from the Vmatch packages which is able to calculate persistent suffix arrays in very short time and with very little memory usage. Gepard will attempt to use this external binary automatically if it can be located in the programs directory or in the environment variable PATH. If you are using Vmatch with Gepard you may run the low-memory version of Gepard as the mkvtree binary will run outside the Java VM.
Looking for the previous version Gepard 1.30? Here is it: gepard-1.30.zip